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rh hpse  (R&D Systems)


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    R&D Systems rh hpse
    Rh Hpse, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rh hpse/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    rh hpse - by Bioz Stars, 2026-04
    94/100 stars

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    recombinant human rh gusb  (R&D Systems)
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    A) Curcumin-glucuronide specific deconjugation activity of <t>GUSB,</t> HPSE, or KL was determined by quantitation of curcumin-glucuronide substrate and curcumin product after <t>incubating</t> <t>recombinant</t> human enzymes with curcumin-glucuronide (n=3–6/group). **** p < 0.0001 vs control (or as specified). B) Immunoblot of GUSB, HPSE, and KL expression in indicated tissues from female, 4-week, C57BL/6J mice (representative of n=4 blots). C) Tissue-specific GUSB enzyme activity using 4-MUG in 4-week female C57BL/6J mice (n=4–5/group). ****p < 0.0001 vs marrow.
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    A) Curcumin-glucuronide specific deconjugation activity of GUSB, HPSE, or KL was determined by quantitation of curcumin-glucuronide substrate and curcumin product after incubating recombinant human enzymes with curcumin-glucuronide (n=3–6/group). **** p < 0.0001 vs control (or as specified). B) Immunoblot of GUSB, HPSE, and KL expression in indicated tissues from female, 4-week, C57BL/6J mice (representative of n=4 blots). C) Tissue-specific GUSB enzyme activity using 4-MUG in 4-week female C57BL/6J mice (n=4–5/group). ****p < 0.0001 vs marrow.

    Journal: Journal of natural products

    Article Title: Beta-glucuronidase catalyzes deconjugation and activation of curcumin-glucuronide in bone

    doi: 10.1021/acs.jnatprod.8b00873

    Figure Lengend Snippet: A) Curcumin-glucuronide specific deconjugation activity of GUSB, HPSE, or KL was determined by quantitation of curcumin-glucuronide substrate and curcumin product after incubating recombinant human enzymes with curcumin-glucuronide (n=3–6/group). **** p < 0.0001 vs control (or as specified). B) Immunoblot of GUSB, HPSE, and KL expression in indicated tissues from female, 4-week, C57BL/6J mice (representative of n=4 blots). C) Tissue-specific GUSB enzyme activity using 4-MUG in 4-week female C57BL/6J mice (n=4–5/group). ****p < 0.0001 vs marrow.

    Article Snippet: Recombinant human (rh)-GUSB (6144-GH), rh-HPSE (7570-GH), and rh-KL (5334-KL), and recombinant mouse receptor activator of NFkB ligand (RANKL, 462-TEC) were purchased from R&D Systems.

    Techniques: Activity Assay, Quantitation Assay, Recombinant, Western Blot, Expressing

    A) GUSB enzyme activity in marrow (normalized to wild-type BL/6J) in 15-week male mice (n=3–5/group). B) Immunoblot of GUSB, HPSE, and KL expression in marrow from 15-week female wild-type C57BL/6J and partial loss-of-function C3H/HeJ mice, and 15-week male hetero- (+/mps) and homozygous (mps/mps) GUSB deficient mice (representative of n=3 biological replicate blots). C) Aglycone curcumin levels 20 minutes after IP curcumin administration (100 mg/kg) to 15-week female BL/6J, female HeJ mice and male mps/mps mice (n=3–5/group), expressed as % of total curcumin in serum vs. marrow suspensions. There were no significant differences in GUSB activity or aglycone curcumin content based on sex (data not shown). ****p < 0.0001 vs C57BL/6J. nd = not detectable

    Journal: Journal of natural products

    Article Title: Beta-glucuronidase catalyzes deconjugation and activation of curcumin-glucuronide in bone

    doi: 10.1021/acs.jnatprod.8b00873

    Figure Lengend Snippet: A) GUSB enzyme activity in marrow (normalized to wild-type BL/6J) in 15-week male mice (n=3–5/group). B) Immunoblot of GUSB, HPSE, and KL expression in marrow from 15-week female wild-type C57BL/6J and partial loss-of-function C3H/HeJ mice, and 15-week male hetero- (+/mps) and homozygous (mps/mps) GUSB deficient mice (representative of n=3 biological replicate blots). C) Aglycone curcumin levels 20 minutes after IP curcumin administration (100 mg/kg) to 15-week female BL/6J, female HeJ mice and male mps/mps mice (n=3–5/group), expressed as % of total curcumin in serum vs. marrow suspensions. There were no significant differences in GUSB activity or aglycone curcumin content based on sex (data not shown). ****p < 0.0001 vs C57BL/6J. nd = not detectable

    Article Snippet: Recombinant human (rh)-GUSB (6144-GH), rh-HPSE (7570-GH), and rh-KL (5334-KL), and recombinant mouse receptor activator of NFkB ligand (RANKL, 462-TEC) were purchased from R&D Systems.

    Techniques: Activity Assay, Western Blot, Expressing